AS H Hassenin has been working as a Professor in the Department of Animal Medicine, Faculty of Veterinary Medicine at Zagazig University, Egypt.
The objective of this study was to compare a commercially available indirect enzyme-linked immunosorbent assay (ELISA) with\r\nan immune peroxidase monolayer test (IPMA), considered to be a reference test, for the detection of antibodies against Lawsonia\r\nintracellularis in horses. A total of 100 serum samples were tested with both tests at three diff erent dilutions 1:125, 1:150 and 1:175.\r\nWhen sera were tested at 1:125 dilutions, the sensitivity of ELISA was 90% with 94% specifi city. At a dilution of 1:150, the sensitivity\r\nwas 88% and specifi city was 96%. At 1:175 dilutions, the sensitivity and specifi city were 85% and 98%, respectively. Based on these\r\nvalidation results, we believe that commercially available ELISA is a useful alternative for the diagnosis of equine proliferative\r\nenteropathy.
Ghazal Adibmoradi got his PhD in 1995 in Human Anatomy from University of Tehran, Iran. He was the Head of the Pathology Department for 8 years at the same university.\r\nHe has published 4 books in the Human and Animal Pathology and Anatomy. He has also published over 20 international and national articles. He is currently a Professor\r\nat University of Tehran and teaches anatomy.
Hemolytic anemia and resultant hypoxia can cause systematic body dysfunctions. Iron delivered from hemolysis of erythrocytes\r\ncan also stimulate oxidative stress. To infer the eff ect of hemolytic anemia on morphological features, the changes of renal cortex\r\nin adult mice was traced through a comprehensive experiment in which four distinct groups containing 7 adult mice were subjected\r\nto experiments. In this context, on the fi rst day normal saline was injected to fi rst group, also referred to as the control group, while 4,\r\n6, and 8 mg/100 g/48 h (i.p.) of Phenylhydrazine were injected to second, third and fourth groups, respectively. From this day forward,\r\nthese quantities were changed to 2, 4, and 6 mg/100 g/48 h (i.p.), respectively. Aft er 35 days, kidneys were fi xed in 10% formal saline\r\nbefore slicing into Paraffi n sections. Aft er tissue processing and sample staining with H&E method, PHZ eff ects in proximal tubule\r\nof renal cortex were determined (P<0.05). It can be concluded that PHZ can disturb the functionality of kidneys by aff ecting the\r\nproximal tubules of renal cortex.