8^th International Conference on Animal Health & Veterinary Medicine October 20-21, 2017 Toronto, Canada (Park Inn by Radisson Toronto Airport West, ON)

AS H Hassenin has been working as a Professor in the Department of Animal Medicine, Faculty of Veterinary Medicine at Zagazig University, Egypt.

The objective of this study was to compare a commercially available indirect enzyme-linked immunosorbent assay (ELISA) with\r\nan immune peroxidase monolayer test (IPMA), considered to be a reference test, for the detection of antibodies against Lawsonia\r\nintracellularis in horses. A total of 100 serum samples were tested with both tests at three diff erent dilutions 1:125, 1:150 and 1:175.\r\nWhen sera were tested at 1:125 dilutions, the sensitivity of ELISA was 90% with 94% specifi city. At a dilution of 1:150, the sensitivity\r\nwas 88% and specifi city was 96%. At 1:175 dilutions, the sensitivity and specifi city were 85% and 98%, respectively. Based on these\r\nvalidation results, we believe that commercially available ELISA is a useful alternative for the diagnosis of equine proliferative\r\nenteropathy.

Ghazal Adibmoradi got his PhD in 1995 in Human Anatomy from University of Tehran, Iran. He was the Head of the Pathology Department for 8 years at the same university.\r\nHe has published 4 books in the Human and Animal Pathology and Anatomy. He has also published over 20 international and national articles. He is currently a Professor\r\nat University of Tehran and teaches anatomy.

Hemolytic anemia and resultant hypoxia can cause systematic body dysfunctions. Iron delivered from hemolysis of erythrocytes\r\ncan also stimulate oxidative stress. To infer the eff ect of hemolytic anemia on morphological features, the changes of renal cortex\r\nin adult mice was traced through a comprehensive experiment in which four distinct groups containing 7 adult mice were subjected\r\nto experiments. In this context, on the fi rst day normal saline was injected to fi rst group, also referred to as the control group, while 4,\r\n6, and 8 mg/100 g/48 h (i.p.) of Phenylhydrazine were injected to second, third and fourth groups, respectively. From this day forward,\r\nthese quantities were changed to 2, 4, and 6 mg/100 g/48 h (i.p.), respectively. Aft er 35 days, kidneys were fi xed in 10% formal saline\r\nbefore slicing into Paraffi n sections. Aft er tissue processing and sample staining with H&E method, PHZ eff ects in proximal tubule\r\nof renal cortex were determined (P<0.05). It can be concluded that PHZ can disturb the functionality of kidneys by aff ecting the\r\nproximal tubules of renal cortex.

Ghazal Adibmoradi recieved his PhD in 1995 in Human Anatomy from University of Tehran, Iran. He was the Head of the Pathology Department for 8 years at the same\r\nuniversity. He has published 4 books in the Human and Animal Pathology and Anatomy. He has also published over 20 international and national articles. He is currently a\r\nProfessor at University of Tehran and teaches anatomy.

In the last decades, the role of PRP (platelet-rich plasma) for the treatment of pattern hair loss has been demonstrated in many\r\nreports. PRP is a biological product that the therapeutic results have proven a signifi cant diff erence compared with synthetic drugs\r\nand some traditional methods, in sports medicine, dermatology and beauty, healing of wounds. In this study, we aimed to evaluate\r\nthe histomorphometrical properties of adult rat skin aft er injection of plasma. Th irty adult rats were divided into 3 groups. First\r\ngroup was control and treated with normal saline, and test groups were nominated as 2 and 3 and were treated with PPP and PRP. Th e\r\nblood samples were collected through cardiac puncture and were centrifuged. Th e upper layer consisted of platelet-poor plasma and\r\nlower layer consisted of platelet-rich plasma. Th e skin samples staining with H&E and Masson-trichrome-methods. Th e thickness\r\nof epidermis, dermis and hypodermis layers, thickness of epithelial hairs root sheath, maximum depth of hair follicles, thickness of\r\nthe whole skin, the number of sebaceous glands and hair follicles in the specifi ed scale, fi nally the number of Fibroblast cells and\r\nthe percent distribution of connective tissue fi bers were investigated in histometrical structure of the skin and skin appendages. Th e\r\nresults of this study indicated that systematic and controlled PRP may have benefi cial eff ects on the reconstruction and rehabilitation\r\nof the skin and its appendages. Present study showed that growth factors in plasma especially PRP, must have stimulated connective\r\ntissue fi broblasts of the dermal layer of the skin.

Shigeru Sato earned his MS degree in Pathology of Bovine Leukemia from Iwate University in 1980, thereafter he worked for Miyagi Prefectural Federation of Agricultural\r\nMutual Aid Association from 1980-2006. He earned his PhD in Ruminal Bacteria and Immune Response of Calves from Tohoku University in 1991, and also in Immunity of\r\nPeriparturient Cows from Kitasato University in 1998. Now, he is a faculty member of Iwate University, and teaches a course on food animal internal medicine and bovine\r\nclinics.

Changes in energy and health statuses were evaluated in calves with short-term fasting and debilitating diarrhea to investigate the\r\neff ects of oral administration of glycerol. Six Jersey calves (male, 2-4 months of age) were divided into three groups, and glycerol\r\nwas orally administered to calves (200 ml, 50 ml, and 0 ml as the control) by catheter. Th e experiment was repeated three times.\r\nBlood glucose concentration peaked at 2 hours aft er administration, and it lasted until 6 hours aft er administration. Increase in blood\r\nglucose, decrease in FFA, and these durations were facilitated concurrently with the increase in volume of glycerol administration.\r\nTh erefore, energy supplementation might be achieved by increasing blood glucose followed by oral administration of glycerol (50-200\r\nml) in calves with 2-4 months of age. On the other hand, debilitative fi ndings in diarrheal calves (n=11), such as decrease and absence\r\nof activity, dysstasia and unstable gait, and vitality, were improved in 10 calves, and dysstasia and staggering were recovered in 3 calves\r\nby 200 ml glycerol administration using catheter. Furthermore, feces color (n=3), physical nature (n=8), and odors (n=3) were also\r\nimproved on the following day of glycerol administration. From these fi ndings, it was revealed that glycerol administration of 200 ml\r\nto debilitated calves by chronic diarrhea might accelerate the recuperation from the debilitation and diarrhea.