Ashraf M A Barakat
National Research Centre, Egypt
Title: Public health hazard and risk assessment of zoonotic campylobacter jejuni reference to some governorates in Egypt
Biography
Biography: Ashraf M A Barakat
Abstract
Several Campylobacter species are known to be pathogenic to humans, with Campylobacter jejuni being the main leading cause of campylobacteriosis worldwide. The present investigation aimed to detect C. jejuni from chicken, water, milk and milk products and humans among 4 Egyptian Governorates (Cairo, Fayoum, Minya and Qalubiya) using conventional method and PCR. This study spots light on this concept to indemnify zoonotic hazard of C. jejuni by molecular characterization and indirect fluorescent of Egyptian isolates from both chickens and human in contact. From various Egyptian governorates. Samples from both symptomatic consumers with history of chickens poisoning and chicken handler. Also Samples as water, milk and milk products were subjected to standard phenotypic identification of C.jejuni, and subsequently immunofluorescent technique (IFT) identification and genetic amplification by PCR using specific primers of hipO gene. It is clear that C. jejuni could be detected from chicken (38.6 %), human stool (33.3%), water samples (30%), total milk and milk products samples (7.9%). While the percentages were chicken (80 %), water (76.7 %), milk and its products (72.5 %) and human (76.3 %) usig PCR. PCR was definitive, reliable, and facilitated rapid identification of C. jejuni to the species level. It concluded that poor hygiene and sanitation in poultry farms could explain this high level of prevalence of C. jejuni among the examined samples. A high risk score for Campylobacter jejuni in chicken was obtained in Egypt . This result may contribute to development of food safety management strategies. Key efforts should be made to control the risk of Campylobacter jejuni in chicken and milk and milk products in Egypt., especially in chick breeding and chicken preparation processes.