Mai Abuowarda
Cairo University, Egypt
Title: Immunoprotective efficacy of a purified midgut antigens of hyalomma anatolicum anatolicum in Egypt
Biography
Biography: Mai Abuowarda
Abstract
H. a. anatolicum was collected from naturally infested cattle in Baharia, Al Kharga Oases and Siwa (Egypt). Midgut antigens were purified in to three peaks (Peak I, peak II and peak III) by gel filtration chromatography for immunization of rabbits against tick infestation. Purified midgut peak III was a significant reduction in the biological parameters of tick and effect in feeding and reproduction performance of tick. Serum globulins were a significant (P > 0.05) increase in rabbits immunized with peak II then peak III of purified midgut. Antibody levels were detected by ELISA with a significant (P > 0.05) increase in rabbits with peak III. Detection of polypeptide bands by using SDS-PAGE. By using western blot, identified a 59, 51, 33 and 20KDa protein might be diagnostic specific in purified midgut (peak II &peak III) of H. a. anatolicum ticks while the polypeptide band with molecular weight 66 KDa was the most immunogenic epitopes might be play role in immunization and protection against H. a. anatolicum in Egypt.