Soad M. Nasr
National Research Centre, Egypt
Title: Biochemical and hormonal changes in serum of dairy cattle suffered from infertility
Biography
Biography: Soad M. Nasr
Abstract
The present study was conducted to evaluate the importance of some metabolic profile tests in diagnosis and prognosis of infertile cows suffering from smooth inactive ovaries and cystic ovaries. In addition to determining serum biochemical and hormonal changes associated with these problems. The main complaint was delayed estrus and lower conception rate. Twenty-three dairy cows were divided into three groups according to rectal palpation selected from different localities at El-Farafra and El-Dakhla Oases, New Valley governorate. The first group (n=12) showed small and smooth ovaries (clinically showing anestrum). The second group (n=5) had cystic ovaries (showed repeat breeder). The last two infertile groups fed Berseem hegazy (Alfalfa) only or Berseem hegazy and Boos or Tibin. The third group, control healthy, fertile cows (n=6) exhibited a physiological estrous cycle and showing regular conception rate. They were fed balanced ration (Berseem; Trifolium alexanderinum), concentrates, corn, tibin. Blood samples were collected from jugular vein puncture from all animals for determination of serum biochemical parameters, micro- and macro- elements and hormonal analyses. Representative samples of soil, plants and wells' water from different studied localities were collected for determination of available phosphorus, iron, zinc, manganese and copper. Estimation of electrical conductivity in the water was also done. Analysis of the data revealed that anestrum cows had significantly low levels of phosphorus, sodium, copper, selenium and iron. However, cows suffered from cystic ovaries had significantly low phosphorus, selenium and iron levels. The two infertile groups had significantly higher serum calcium/phosphorus ratio, aspartate aminotrasferase activity and estrogen. There was a marked decrease in serum total proteins, blood urea nitrogen, progesterone concentrations in both infertile groups. Serum cholesterol, T3 and T4 were markedly decreased, while serum triglycerides were increased in cystic ovaries group. Analysis of the soil and plants revealed low phosphorus concentrations and high levels of some trace elements' content. In conclusion, absence of balanced ration, deficiency of phosphorus in soil and feed, and presence of phytoestrogenic isoflavones led to dysfunction of the ovaries in the examined dairy cows with subsequent low in conception rate and high incidence of infertility. Soil must be treated with phosphate fertilizers. Berseem hegazy must be supported by adding concentrates and mineral mixture. containing sodium dihydrogen phosphate, selenium and vitamins.