Eman H Abdel-Rahman
National Research Centre, Egypt
Title: A 30 KDa mannosyle glycoprotein in the diagnosis of experimental trichinosis in rats
Biography
Biography: Eman H Abdel-Rahman
Abstract
A method of affinity chromatography was adopted to isolate mannosyl glycoprotein antigen from Trichinella spiralis crude larval antigen using concanavalin A (Con A) column. The selection of con A column was based on the existence of mannose sugar in T.spiralis larval extract as proved by indirect haemagglutination inhibition assay. The isolated mannosyl fraction was evaluated by ELISA and Western blot techniques for the serological diagnosis of trichinosis in experimentally infected rats. Either early in one week post infection (P.I.) or in the late stage, six weeks P.I., the isolated mannosyl fraction proved higher potency in the diagnosis of rat experimental trichinosis than crude extract by ELISA. The isolated mannosyl fraction resolved into four bands of 65KDa, 54KDa, 30 KDa and 11KDa compared with multiple bands of crude extract as observed by SDS-PAGE. A 30 KDa mannosyle glycoprotein(s) isolated from T.spiralis crude larval extract proved potentials in early as well as late diagnosis of experimental trichinosis in rats as proved by western blot.
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