Animal Health & Veterinary Medicine

The aim of this study was to investigate the effect of isoflurane on total antioxidant capacity in calves. The research was carried out on 15 calves, from newborns to 3-months-old, in operations performed for a variety of reasons.  For induction, isoflurane was given at 3-5% concentration via mask during 15 minutes. Then, endotracheal intubation was performed and the maintenance was set to a concentration of 1.5-3% isoflurane in 100% oxygen two hours period. Blood samples were taken at 30 and 75 minutes, after the induction, before of anaesthesia, during two hours isoflurane anaesthesia and total antioxidant capacity were evaluated. Blood samples were centrifuged at 3500 rpm for 10 minutes at +4°C and the serum samples were obtained. The samples were maintained at -20°C until analyses. Total antioxidant capacity (TAC) was determined using commercial kits using a spectrophotometer (Eo Biotex, USA). There was no significant difference recorded on TAC during isoflurane anaesthesia. It was determined the isoflurane anesthesia had no adverse effect on total antioxidant capacity in calves.