Mian A Hafeez
University of Veterinary and Animals Sciences, Pakistan
Title: Genome structure sharing by reptiles and avians as of Isospora from bearded dragon and black throated laughing thrush
Biography
Biography: Mian A Hafeez
Abstract
The complete mitochondrial genome of Isospora amphiboluri and Isospora sp. n. (Eimeriidae, Coccidia, Apicomplexa) were sequenced. These coccidia infect the bearded dragon, Pogona vitticeps (Sauria: Agamidae) and black throated laughing thrush Garrulax chinensis (Aves: Passeriformes) respectively. PCR was performed post DNA extraction and protein estimation by Nanodrop. The Whole Genome was achieved in fragments and assembled. Genome organization and gene content was conventional. The circular-mapping mt genome of Isospora amphiboluri is 6264bp and Isospora sp. n. 6158bp in length consisting of 3 protein coding genes (cox1, cox3 and cytb), 19 gene fragments encoding large subunit (LSU) rRNA and 14 gene fragments encoding small subunit (SSU) rRNA. Like other Apicomplexan, no tRNA was encoded. The comparable mitochondrial genome sequences and structures of Isospora and Eimeria species confirm the close relationship between these eimeriid genera of apicomplexan parasites. Investigation of Eimeria genes intricate the basic biology and host-parasite interaction and highlights adaptations to a comparatively simple developmental life cycle. The purpose of this study was to sequence and annotate the complete mitochondrial genomes of Isospora amphiboluri and Isospora sp. n that commonly infect bearded dragon and black throated laughing thrush.