Hamouda A G
Cairo University, Egypt
Title: Molecular characterization of campylobacter jejuni and campylobacter coli in chicken in Egypt
Biography
Biography: Hamouda A G
Abstract
Campylobacter lives in the intestines of birds (specifically poultry) and other animals. It can be found in water, food, or surfaces that have been contaminated with the feces of infected birds. This work was done to investigate the molecular characterization of C. jejuni and C. coli isolated from rectal swabs of chicken, intestinal content, gizzard, liver, eggs, water and ration from chicken farms. We collected 498 samples from chicken (n=143) broilers and (n=355) layers at different localities. All samples were examined by conventional methods for isolation and identification of Campylobacters. Samples were subjected to standard phenotypic identification of C.jejuni and C. coli by PCR using specific primers of hippuricase gene. The prevalence of Campylobacter isolate was 21.89%; in rectal swabs 23.77%, intestine 24 %, liver 23 %, 21.48% in eggs, 12% water and 6.67% in ration respectively. Out of 109 identified isolates, 81(16, 27%) C. jejuni isolates were higher than 28(5.62%) C.coli. A multiplex-PCR method was developed for the detection of C. jejuni and C. coli. Primers were the hippuricase gene (hipO) C. jejuni at 323bp and (C. coli glyA) characteristic of C.coli at 126bp in chicken isolates in Egypt. The virulence genes of C. jejuni (FlaA, virB11) have shown at 855 bp and 494 bp respectively. But the cytolethal distending toxin (cdtB and cdtC) of C. jejuni have shown at 495 bp and 555 bp respectively, indicating the virulence of the isolates. The results of the present study showed that all analyzed isolates of C. jejuni and C. coli contained the flaA gene. On the other hand, the virB11 gene was present only in 10 of 81 of the analyzed isolates, whereas most of the strains contained the cdt genes. Finally, we concluded that PCR detection of Campylobacter virulence can be utilized as a simple and rapid tool to discriminate stains and we need devise effective therapeutic and preventive strategies against Campylobacter infection in chicken.