Mona S Mahmoud
National Research Centre, Egypt
Title: The immunization of sheep against Babesia ovis with purified fractions of parasitized erythrocytes
Biography
Biography: Mona S Mahmoud
Abstract
The present study was undertaken in an effort to furnish information and materials for investigating control of babesiosis in sheep. Sonicated erythrocytes infected with Babesia ovis Egyptian strain was subjected to ion exchange chromatography. The proteins were fractionated into two purified fractions (unbound & bound). Sodium dodecyl sulphate poly-acrylamide gel electrophoresis (SDS-PAGE) fractionation of the crude sonicated antigen and the two purified fractions showed obvious qualitative and quantitative differences. The two purified fractions were used as immunogens for two groups of sheep using Freund's adjuvants in two successive doses. The two immunized groups plus a control one were challenged with 2.5 X 105 cryo-preserved infected erythrocytes with a homologous strain of Babesia ovis. Blood samples were collected for assessment of packed cell volume and parasiteamia percentage. The indirect fluorescent and immuno-blotting techniques were used for evaluation of immunization process. All animals in immunized groups were protected against infection, when compared with those in control group.
