5^th Animal Health and Veterinary Medicine Congress

Biography

Biography: Imre Olah

Abstract

In avian species the bursa of Fabricius is a primary lymphoid organ, which is responsible for B cell differentiation and antigen- specific IgY production. The surface epithelium of the bursa comprises approximately 90% interfollicular and 10% follicle-associated epithelium. The interfollicular epithelium has a discontinuous basal cell layer that may be differentiated to interfollicular epithelial cells secreting mucin-like substance. The early experimental studies suggested that the endocytosis of follicle-associated epithelium provides antigen access from the bursal lumen into the follicles. The aim of this study is to investigate the Caveolin-1 mediated endocytosis of interfollicular epithelium in chicken’s bursa of Fabricius. The immunohistochemistry of Caveolin-1 indicated that no Caveolin-1 mediated endocytosis in the follicle-associated epithelium, but in the interfollicular epithelium Caveolin-1 positive and negative cells were found. The transmission electron microscopy confirmed the two types of interfollicular epithelial cells. After infectious bursal disease virus infection the number of Caveolin-1 positive cells drastically decreased, but by 28 days postinfection their number returns to that of the control. These results indicate that not only the follicle-associated but the interfollicular epithelium also participates in the Gumboro infection and this is the first paper dealing with the Caveolin-1 expression in the interfollicular epithelium after Gumboro virus infection.